RNA

Part:BBa_K3875023

Designed by: Yimiao Lin   Group: iGEM21_BUCT   (2021-10-01)


Usage and Biology

23: sgRNA-R Using a strand of single guide RNA (sgRNA), the Cas9 protein is able to search a cellular genome and induce double stranded breaks at a target sequence complementary to the sgRNA that can then be modified.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None